NEW RUN
Sequence submission
Analysis scope
22-nt spacers · 5-nt PFS · Exact mismatch counts from 0–8
PFS scores are bacterial-screen measurements. Sequence distance is a screening filter, not a validated safety score. Reference matches include intended human targets and precautionary reverse-complement matches.
Nucleotides
Scanned candidates
Pass distance filter
1
Ranked guide candidates
| Rank | Position | Spacer RNA, 5′ → 3′ | PFS | PFS score | Closest active site |
|---|
No candidates pass the selected distance filter.
| Mismatches | Sense sites | Sense: active PFS | Reverse-complement sites | Reverse: active PFS |
|---|